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| Method | Hanging-drop vapour diffusion |
| Plate type | 24-well plates (Hampton Research) |
| Temperature (K) | 293 |
| Protein concentration (mgml1) | 89 |
| Buffer composition of protein solution | 20mM TrisHCl pH 8.0, 150mM NaCl |
| Composition of the reservoir solution | 0.6M ammonium phosphate, 0.1M imidazole pH 8.2 |
| Volume and ratio of drop | 2l, 1:1 |
| Volume of reservoir (l) | 400 |
