| Method |
Hanging-drop vapour diffusion |
Hanging-drop vapour diffusion |
| Plate type |
VDX plate (Hampton Research) |
VDX plate (Hampton Research) |
| Temperature (K) |
295 |
295 |
| Protein concentration (mgml1) |
50 |
35 |
| Buffer composition of protein solution |
20mM HEPES pH 7.5, 150mM NaCl, 1mM EDTA |
20mM HEPES pH 7.5, 150mM NaCl |
| Composition of reservoir solution |
100mM HEPES pH 7.5, 16% PEG 4000, 250mM ammonium sulfate, 12% 2-propanol |
170mM lithium sulfate, 25% PEG 3350 |
| Volume and ratio of drop |
2l, 1:1 |
2l, 1:1 |
| Volume of reservoir (l) |
1000 |
1000 |
