Table 2. Crystallization.
Method | Hanging-drop vapour diffusion |
Plate type | 96-well Art Robbins CrystalMation Intelli-Plate (Hampton Research) |
Temperature (K) | 293 |
Protein concentration (mgml1) | 10 |
Buffer composition of protein solution | 100mM ammonium acetate, 12%(w/v) PEG 3350, 50mM bis-tris pH 5.5 |
Composition of reservoir solution | 200mM ammonium acetate, 23%(w/v) PEG 3350, 100mM bis-tris pH 5.5 |