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. 2015 Jan 23;46(1):5. doi: 10.1186/s13567-014-0132-5

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© Sadeyen et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Levels of cytokine transcripts in the spleens of vaccinated turkeys as measured by real-time qRT-PCR. Results are expressed as corrected ΔCt values ± standard error of the mean after normalization with the Ct value of 28S rRNA product of each sample. Turkey poults were given sterile saline (mock) as a coarse spray (open bars), a formalin-inactivated vaccine based on the parent strain EC34195 formulated in aluminium hydroxide adjuvant via the subcutaneous route (black bars) or Poulvac® E. coli as a coarse spray (checked black and white bars) starting at one day-old. Five birds from each group were sampled per time-point. *denotes P ≤ 0.05 relative to mock-vaccinated controls.