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| Primer | Adapter | Key | MID | Specific oligonucleotide * (5’ → 3’) |
|---|---|---|---|---|
| B1F-fusion (forward) | CCATCTCATCCCTGCGTGTCTCCGAC | TCAG | Variable (10-mers) | CCGGCGCCGACHTNTTYTAYTT |
| D4R-fusion (reverse) | CCTATCCCCTGTGTGCCTTGGCAGTC | TCAG | None | CAGCCGGGTCTCGATCCAYTCNCC |
*Specific oligonucleotides consist of a degenerate ‘core’ (plain text) and non-degenerate ‘clamp’ (bold) region.
