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. 2015 Jan 23;10(1):e0117357. doi: 10.1371/journal.pone.0117357

Figure 3. HeLa cell growth is not inhibited by a knockdown-deficient PAK3 shRNA.

Figure 3

PAK3 mRNA levels were determined by quantitative reverse transcription PCR analysis at 72 hours after infection with the respective shRNA viruses. Live cell numbers were counted 5 days after the addition of 20 ng/mL doxycycline. The bar graph represents the average ± standard deviation of 3 replicates from one of two independent experiments with similar results. PAK3 mRNA levels were normalized with GAPDH expression; (A) Effect of wild-type (3245) and mutant PAK3 shRNA 3245 (3245 m15) on PAK3 mRNA levels in HeLa cells; (B) Effect of wild-type (3245) and mutant PAK3 shRNA 3245 (3245 m15) on HeLa cell growth.