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. Author manuscript; available in PMC: 2015 Jul 22.
Published in final edited form as: Nature. 2014 Nov 5;517(7535):497–500. doi: 10.1038/nature13896

Extended Data Fig. 1. mTORC1 is activated following Wnt-signal and its inhibition does not affect homeostasis.

Extended Data Fig. 1

a) Representative IHC of phospho-rpS6 and phospho-4EBP1 show mTORC1 activity during intestinal regeneration, 72hrs after 14Gy γ-irradiation (representative of 5 biological replicates); b+c) boxplots demonstrating that 72hrs of 10mg/kg rapamycin treatment does not alter mitosis or apoptosis in normal intestinal crypts. Whiskers are max and min, black line is median (n=4 per group; NS: not significant, Mann-Whitney U test); d) Intestines imaged on OV100 microscope, 96hrs post induction, for RFP. Tissue without the ROSA-tdRFP reporter (labelled Neg Control) show no RFP positivity, while the positive control and Raptor fl/fl intestines show high RFP positivity (representative of 3 biological replicates).; e+f) boxplot showing that Raptor deletion does not affect mitosis or apoptosis rates in intestinal crypts, 96hrs after induction. Whiskers are max and min, black line is median (n=4 per group; NS: not significant, Mann-Whitney U test). Scale bar = 100μm