Skip to main content
. 2015 Jan 23;10(1):e0116917. doi: 10.1371/journal.pone.0116917

Figure 2. Transformation of the H9N2/SA-HA and H5N1/VSVRI-HA cloned in standard pHW2000 reverse genetic vector using recA13/HB101.

Figure 2

A) and C): Colonies with large and small phenotype were detected (arrow indicating the small type) after transforming the recA13/HB101 with pHW2000 encoding the DNA insert of H9N2/SA- or H5N1/VSVRI-HA segment, respectively. B) and D): Restriction analysis of pHW-H9N2/SA-HA and pHW-H5N1/VSVRI-HA plasmid DNA isolated from the HB101 transformants showed the expected digestion pattern only with small-sized colonies and an incorrect pattern with all large-sized colonies.