Table 4. Characteristics of sperm motility in the frozen-thawed capercaillie semen depending on freezing method (means ± SD).

Male’s number	Freezing methods	No of samples	Motile sperm [%]	Motility parameters
				VCL [µm s-1]	VSL [µm s-1]	VAP [µm s-1]	LIN [%]	ALH [µm]
51	pellets	12	31.0 ab ±13.5 x	44.3 bc ±6.5	15.3c±1.3 x	25.4b±3.8	34.9 bcd ±2.4 x	3.5 a ±1.4 x
	straws		54.5 a ±11.9 y	39.7 c ±5.5	20.3 ab ±5.4 y	27.8 ab ±4.8	51.5 a ±13.1 y	2.5 a ±0.5 y
58	pellets	9	13.9 c ±8.6 x	41.0 cd ±3.9	17.4 b ±2.6	26.4 b ±3.3	40.1 b ±6.3	2.1 cd ±0.3
	straws		33.3 b ±12.5 y	40.6 c ±2.2	18.7 bc ±4.0	26.3 abc ±2.4	44.0 b ±9.7	2.6 a ±1.0
9	pellets	8	41.7 a ±21.2 x	53.2 a ±10.7 x	19.6 a ±3.2 x	31.9 a ±5.1 x	39.1 bc ±11.2	2.9 b ±0.7 x
	straws		53.3 a ±13.3 y	42.1 bc ±4.3 y	16.2 cd ±1.3 y	25.5 bc ±2.8 y	37.8 c ±8.2	2.3 ab ±0.4 y
49	pellets	11	38.1 ab ±15.8 x	45.4 bc ±5.9	14.5 c ±1.2	25.6 b ±2.8	33.9 cd ±3.2	2.7 b ±0.4
	straws		55.0 a ±11.9 y	44.5 b ±5.5	14.7 d ±1.3	25.2 bc ±3.2	34.7 c ±2.4	2.6 a ±0.4
67	pellets	8	29.0 b ±10.8	47.7 b ±3.8	16.0 bc ±1.0	27.0 b ±1.5	36.2 bcd ±4.9	2.7 b ±0.2
	straws		39.2 b ±14.0	48.5 a ±4.3	15.8 d ±1.2	27.4 ab ±2.0	33.9 c ±4.2	2.8 a ±0.3
72	pellets	12	9.0 c ±5.0 x	39.7 de ±4.7	11.8 d ±0.8 x	21.2 c ±1.5	32.3 d ±4.3	2.4 bc ±0.4
	straws		33.1 b ±12.8 y	41.8 bc ±4.0	14.4 d ±1.6 y	23.8 c ±2.8	36.0 c ±2.0	2.5 a ±0.2
79	pellets	9	15.7 c ±8.7 x	35.8 e ±1.3 x	20.2 a ±3.0	25.6 b ±2.2 x	53.1 a ±6.3	1.7 d ±0.2
	straws		38.0 b ±15.6 y	42.9 bc ±2.1 y	22.3 a ±3.6	28.6 a ±2.8 y	50.4 a ±7.8	2.0 b ±0.2
Total/Average	pellets	69	27.4±16.5 x	43.9±7.5	16.3±3.3	26.1±4.0	38.3±8.5	2.6±0.7
	straws		46.3±15.9 y	43.1±4.7	17.4±3.9	26.4±3.2	40.9±9.7	2.5±0.5

^a,b,c,d,e—means in columns for the same freezing method followed by different superscripts differ significantly [P<0.05];

^x,y—means in columns for the same male indicate significant differences between freezing method [P<0.05].