Table 1. Summary of animal, immunohistochemical and serial protein misfolding cyclical amplification data.
| Deer ID | Sex | Challenge status | DPC-PME | Genotype (PrP gene, codon 226) | IHC for PrPd | sPMCA 1st Exp. (3rd round) | sPMCA 2nd Exp. (3rd round) | sPMCA 2nd Exp. (4rd round) |
|---|---|---|---|---|---|---|---|---|
| 322 | M | UNC | 190 | N | 0/4 | 0/4 | 0/4 | |
| 319 | M | UNC | 365 | QE | N | - | - | - |
| 334 | M | UNC | 365 | EE | N | 0/4 | 0/4 | 0/4 |
| 032 | F | UNC | 2320 | N | - | - | - | |
| 034 | F | UNC | 2320 | QE | N | - | - | - |
| 039 | F | UNC | 2320 | EE | N | 1/4 | 0/4 | 0/4 |
| 014 | F | UNC | 2320 | EE | N | - | - | - |
| 327 | M | BSE-C | 190 | QE | N | - | - | - |
| 309 | M | BSE-C | 190 | EE | N | - | - | - |
| 031 | F | BSE-C | 190 | N | - | - | - | |
| 320 | M | BSE-C | 190 | QE | N | - | - | - |
| 324 | M | BSE-C | 190 | N | - | - | - | |
| 326 | M | BSE-C | 190 | N | - | - | - | |
| 323 | M | BSE-C | 365 | QE | N | 0/4 | 0/4 | 0/4 |
| 337 | M | BSE-C | 365 | EE | N | 0/4 | 0/4 | 0/4 |
| 314 | M | BSE-C | 365 | N | 0/4 | 0/4 | 0/4 | |
| 318 | M | BSE-C | 365 | QE | N | - | - | - |
| 310 | M | BSE-C | 365 | QE | N | - | - | - |
| 028 | F | BSE-C | 365 | N | 0/4 | 0/4 | 0/4 | |
| 009 | F | BSE-C | 1727 | P | 4/4 | 4/4 | 4/4 | |
| 015 | F | BSE-C | 2320 | QE | N | 0/4 | 0/4 | 0/4 |
| 033 | F | BSE-C | 2320 | EE | N | 0/4 | 0/4 | 0/4 |
| 016 | F | BSE-C | 2320 | EE | N | 0/4 | 0/4 | 0/4 |
| 023 | F | BSE-C | 2320 | QE | N | 0/4 | 0/4 | 0/4 |
| 037 | F | BSE-C | 2320 | EE | N | 0/4 | 0/4 | 0/4 |
Deer identification, sex, challenge status, time of post-mortem examination, genotype at codon 226 of prion protein gene, presence of abnormal protein by immunohistochemistry (PrPd) and western blotting (PrPres) by western blotting after different experiments of serial protein misfolding cyclic amplification (sPMCA). The animal positive for abnormal prion protein is deer 009 and this was by both IHC and sPMCA. Note that for sPMCA a value of 1/4 is considered negative and probably due to contamination of the sample in this exceptionally sensitive technique. DPC-PME = days post challenge of post mortem examination, PrP = prion protein, PrPd/PrPres = abnormal prion protein, IHC = immunohistochemistry, sPMCA = serial protein misfolding cyclic amplification, Exp. = experiment, M = male, F = female, UNC = unchallenged negative control, BSE-C = BSE oral challenged, Q = glutamine, E = glutamic acid, N = negative, P = positive, for sPMCA x/x = number of PrPres positive tubes/number of replicates, – = not examined by sPMCA.