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. 2015 Jan 23;10(1):e0116857. doi: 10.1371/journal.pone.0116857

Figure 6. Phylogenetic relationship between A. thaliana and H. brasiliensis latex ABCG proteins.

Figure 6

The amino acid sequences of all the A. thaliana ABCG proteins and H. brasiliensis latex proteins were aligned using the MUSCLE program and subjected to phylogenetic analysis by the distance with neighbor-joining method using MEGA 5.05 software. The numbers on the nodes indicate the bootstrap values after 1000 replicates. The scale bar indicates the estimated number of amino acid substitutions per site. Accession numbers for the A. thaliana sequences are AtABCG1 (NP_181467.1), AtABCG2 (NP_181272.1), AtABCG3 (NP_850111.1), AtABCG4 (NP_194305.1), AtABCG5 (NP_178984.1), AtABCG6 (NP_196862.1), AtABCG7 (NP_178241.1), AtABCG8 (NP_200098.1), AtABCG9 (NP_194472.3), AtABCG10 (NP_175734.1), AtABCG11 (NP_173226.2), AtABCG12 (NP_175561.1), AtABCG13 (NP_175557.1), AtABCG14 (NP_564383.1), AtABCG15 (NP_188746.2), AtABCG16 (NP_191069.2), AtABCG17 (NP_191070.1), AtABCG18(NP_191071.1), AtABCG19 (NP_191073.1), AtABCG20 (NP_190919.1), AtABCG21 (NP_189190.2), AtABCG22 (NP_568169.1), AtABCG23 (NP_197442.1), AtABCG24 (NP_175745.4), AtABCG25 (NP_565030.1), AtABCG26 (NP_187928.2), AtABCG27 (NP_190799.2), AtABCG28 (NP_200882.4), AtABCG29 (NP_566543.1), AtABCG30 (NP_193258.3), AtABCG31 (NP_180555.2), AtABCG32 (NP_180259.1), AtABCG33 (NP_181265.1), AtABCG34 (NP_181179.2), AtABCG35 (NP_172973.1), AtABCG36 (NP_176196.1), AtABCG37 (NP_190916.1), AtABCG38 (Q7PC85.1), AtABCG39 (NP_176867.2), AtABCG40 (NP_173005.1), AtABCG41 (NP_680692.1), AtABCG42 (NP_680693.5) and AtABCG43 (NP_680694.2). AtSKD1 (AEC08019.1) and HbSKD1 (AIN75626.1) were used as outgroups. The H. brasiliensis latex ABC proteins are marked with a dot.