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. 2015 Jan 23;10(1):e0116857. doi: 10.1371/journal.pone.0116857

Figure 8. Regulatory effects of ethylene and JA on the gene expressions of the four HbABC transporters in the H. brasiliensis latex.

Figure 8

Mature, virgin (untapped) rubber trees were treated with Ethrel (ET) or methyl-jasmonate (Me-JA) for 0.5, 1.5, 4.0, 8.0 or 24.0 h. The controls were rubber trees that had not been treated with stimulants. Three independent biological replicates were included in each sample. Fresh latex from each sample was collected and used to isolate the total RNA. The transcript abundance of each gene was detected by RT-qPCR and the values are shown as the mean ± S.D. Statistical significance was determined using Students t-test using SPSS 19.0 software (Chicago, U.S.A.). When compared with the control, one asterisk shows a significant difference with a P-value < 0.05 and two asterisks show a very significant difference with a P-value < 0.01.