Table 1. Summary of the hydrodynamic and structural data of mortalin.
Mortalin structural and hydrodynamic properties | Predicted for a sphere † | Experimental determination | ||||
---|---|---|---|---|---|---|
Monomer | Dimer | aSEC | AUC # | SAXS | HydroPro | |
MM (kDa) | 70.7 | 141.4 | 75 ± 4 | 76 ± 4 | 66 ± 2 | - |
s020,w (S) | 6.1 | 9.6 | - | 4.8 ± 0.1* | - | 4.9 ± 0.2 |
Rs (Å) | 27 | 34 | 35 ± 2 | - | - | 34 ± 3 |
ƒ/ƒ0 | - | - | 1.30 ¥ | 1.36 ± 0.01 | - | 1.30 |
Rg (Å) | - | - | - | - | 36 ± 2 § 38 ± 2 & | 33 ± 2 |
Dmax (Å) | - | - | - | - | 130 ± 10 | 116 ± 3 |
† Values predicted as globular monomers in water and 20°C (predicted by the Sednterp software)
# calculated from SedFit from the sedimentation velocity data;
* data extrapolated for water, 20°C and 0 mg.mL-1 of protein;
§ data from Guinier law;
& data from p(r) curve;
¥ data obtained using the Stokes equation for proteins of known Rs (predicted by Sednterp software).