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. 2015 Jan 23;10(1):e0117170. doi: 10.1371/journal.pone.0117170

Table 1. Summary of the hydrodynamic and structural data of mortalin.

Mortalin structural and hydrodynamic properties Predicted for a sphere Experimental determination
Monomer Dimer aSEC AUC # SAXS HydroPro
MM (kDa) 70.7 141.4 75 ± 4 76 ± 4 66 ± 2 -
s020,w (S) 6.1 9.6 - 4.8 ± 0.1* - 4.9 ± 0.2
Rs (Å) 27 34 35 ± 2 - - 34 ± 3
ƒ/ƒ0 - - 1.30 ¥ 1.36 ± 0.01 - 1.30
Rg (Å) - - - - 36 ± 2 § 38 ± 2 & 33 ± 2
Dmax (Å) - - - - 130 ± 10 116 ± 3

Values predicted as globular monomers in water and 20°C (predicted by the Sednterp software)

# calculated from SedFit from the sedimentation velocity data;

* data extrapolated for water, 20°C and 0 mg.mL-1 of protein;

§ data from Guinier law;

& data from p(r) curve;

¥ data obtained using the Stokes equation for proteins of known Rs (predicted by Sednterp software).