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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nat Struct Mol Biol. 2014 Nov 24;21(12):1058–1067. doi: 10.1038/nsmb.2922

Figure 5. A Phosphate Tether Incorporated into SAH-MPER(671-683KKK)(q) Engages the Putative Lipid Binding Site.

Figure 5

(a) Position 683 of SAH-MPER(671-683KKK)(q) was derivatized with a Dab-Gly-pSer tether (brown) to span the measured 5.5 Å between the primary amine of Lys683 and the phosphate observed at the 4E10 Fab–SAH-MPER(671-683KKK)(q) interface. (b) Crystal structure of the SAH-MPER(671-683KKK)(q)pSer complex (shown as a green ribbon and wheat transparent Van der Waals surface) bound to 4E10 at 2.68 Å resolution. (c) The 2Fo-Fc electron density map (1σ level) of the antibody-bound SAH-MPER(671-683KKK)(q)pSer construct.