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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Histol Histopathol. 2014 Sep 9;30(2):213–222. doi: 10.14670/hh-30.213

Fig. 2.

Fig. 2

Immunohistochemical staining for FOXO1 (brown) and E-cadherin (red) in placental villi (40× magnification). A. E-cadherin antibody only: E-cadherin clearly distinguishes villous CTB, which are mononuclear cells encircled by red membranous staining from STB, which form a mononuclear syncytium with only the basal boundary of STB stained red. B. FOXO1 antibody only: positive cells display brown nuclear staining. Double immunostaining for FOXO1 and E-cadherin in gestational age matched control case (C), mild PE (D), severe PE (E) and FGR (F) placentas at 31 weeks gestation. Empty arrowheads point to FOXO1-negative CTB; solid arrowheads point to FOXO1-positive CTB nuclei; empty arrows point to FOXO1-negative STB nuclei; and solid arrows point to FOXO1-positive STB nuclei. (G, H) Negative control for ecadherin and FOXO1 antibodies, respectively.×40