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. Author manuscript; available in PMC: 2016 Jan 22.
Published in final edited form as: Chem Biol. 2014 Dec 31;22(1):139–147. doi: 10.1016/j.chembiol.2014.11.011

Figure 2.

Figure 2

Cy5-LVSR-AOMK can distinguish between MALT1-WT and C464A and preferentially binds under activating conditions. MALT1-WT or MALT1-C464A (400 nM) were incubated with Cy5-LVSR-AOMK at different concentrations in buffer with or without citrate for 30 min at 37°C, followed by TCA precipitation and SDS-PAGE. Fluorescence was scanned (A), followed by staining with a coomassie-based protein stain (B). Data are representative of three independent experiments.