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. Author manuscript; available in PMC: 2016 Jan 22.
Published in final edited form as: Chem Biol. 2014 Dec 31;22(1):139–147. doi: 10.1016/j.chembiol.2014.11.011

Figure 3.

Figure 3

Cy5-LVSR-AOMK can detect active MALT1 after overexpression. Different combinations of Bcl10, MALT1-WT or C464A, respectively, were overexpressed in HEK-293A cells. Cells were lysed in the presence of Cy5-LVSR-AOMK (A) or biotin-LVSR-AOMK (B), respectively, precleared, and subjected to SDS-PAGE, fluorescence scanning, and Western blot analysis with the indicated antibodies. E-64 was added or omitted in the lysis buffer to control for cathepsin binding of the probe. Note that endogenous and Flag-tagged versions of the proteins migrate slightly differently during the SDS-PAGE. The asterisks mark non-specific bands. Data are representative of three independent experiments.