Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Mar;74(3):827–831. doi: 10.1073/pnas.74.3.827

Structure of a large segment of the genome of simian virus 40 that does not encode known proteins.

R Dhar, K N Subramanian, J Pan, S M Weissman
PMCID: PMC430491  PMID: 191835

Abstract

The nucleotide sequence of the region of DNA of simian virus 40 extending from 0.595 to 0.790 map unit has been derived. The sequence includes the DNA complementary to the 5' end of early mRNA and to the 5' end of some of the forms of late RNA. Because there are termination codons in all three phases in early and late RNA, there is a sequence of almost 800 nucleotides of simian virus 40 DNA that probably does not code for known viral proteins. The sequence spans the 5' end of the early mRNA at 0.67 map unit and overlaps a species of late RNA whose 5' end is at 0.65 map unit and whose 3' end is at 0.77 map unit. This RNA is retained on oligo(dT)-cellulose columns in high salt concentrations. Analysis of the sequence of late strand RNA suggests that this RNA is not covalently linked to the mRNA that encodes structural proteins. There is another species of late RNA of simian virus 40 whose 5' end is at 0.775 map unit. The nucleotide sequence of this region of simian virus 40 DNA contains several examples of repeated sequences, most of which are located in DNA that does not encode known peptides. These may be analogous to the reiterated sequences that have been described in animal cell DNA.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bertrand K., Squires C., Yanofsky C. Transcription termination in vivo in the leader region of the tryptophan operon of Escherichia coli. J Mol Biol. 1976 May 15;103(2):319–337. doi: 10.1016/0022-2836(76)90315-6. [DOI] [PubMed] [Google Scholar]
  2. Dhar R., Subramanian K., Zain B. S., Pan J., Weissman S. M. Nucleotide sequence about the 3' terminus of SV40 DNA transcripts and the region where DNA synthesis is initiated. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):153–160. doi: 10.1101/sqb.1974.039.01.021. [DOI] [PubMed] [Google Scholar]
  3. Dhar R., Weissman S. M., Zain B. S., Pan J., Lewis A. M., Jr The nucleotide sequence preceding an RNA polymerase initiation site on SV40 DNA. Part 2. The sequence of the early strand transcript. Nucleic Acids Res. 1974 Apr;1(4):595–611. doi: 10.1093/nar/1.4.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dhar R., Zain S., Weissman S. M., Pan J., Subramanian K. Nucleotide sequences of RNA transcribed in infected cells and by Escherichia coli RNA polymerase from a segment of simian virus 40 DNA. Proc Natl Acad Sci U S A. 1974 Feb;71(2):371–375. doi: 10.1073/pnas.71.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lewin B. Units of transcription and translation: sequence components of heterogeneous nuclear RNA and messenger RNA. Cell. 1975 Feb;4(2):77–93. doi: 10.1016/0092-8674(75)90113-0. [DOI] [PubMed] [Google Scholar]
  6. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Marotta C. A., Lebowitz P., Dhar R., Zain B. S., Weissman S. M. Preparation of RNA transcripts of discrete segments of DNA. Methods Enzymol. 1974;29:254–272. doi: 10.1016/0076-6879(74)29026-8. [DOI] [PubMed] [Google Scholar]
  8. Milstein C., Brownlee G. G., Cartwright E. M., Jarvis J. M., Proudfoot N. J. Sequence analysis of immunoglobulin light chain messenger RNA. Nature. 1974 Nov 29;252(5482):354–359. doi: 10.1038/252354a0. [DOI] [PubMed] [Google Scholar]
  9. Proudfoot N. J., Brownlee G. G. Sequence at the 3' end of globin mRNA shows homology with immunoglobulin light chain mRNA. Nature. 1974 Nov 29;252(5482):359–362. doi: 10.1038/252359a0. [DOI] [PubMed] [Google Scholar]
  10. Reich P. R., Forget B. G., Weissman S. M. RNA of low molecular weight in KB cells infected with adenovirus type 2. J Mol Biol. 1966 Jun;17(2):428–439. doi: 10.1016/s0022-2836(66)80153-5. [DOI] [PubMed] [Google Scholar]
  11. Shenk T. E., Carbon J., Berg P. Construction and analysis of viable deletion mutants of simian virus 40. J Virol. 1976 May;18(2):664–671. doi: 10.1128/jvi.18.2.664-671.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sklar J., Yot P., Weissman S. M. Determination of genes, restriction sites, and DNA sequences surrounding the 6S RNA template of bacteriophage lambda. Proc Natl Acad Sci U S A. 1975 May;72(5):1817–1821. doi: 10.1073/pnas.72.5.1817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Subramanian K. N., Pan J., Zain S., Weissman S. M. The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases. Nucleic Acids Res. 1974 Jun;1(6):727–752. doi: 10.1093/nar/1.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Van de Voorde A., Contreras R., Rogiers R., Fiers W. The initiation region of the SV40 VP1 gene. Cell. 1976 Sep;9(1):117–120. doi: 10.1016/0092-8674(76)90057-x. [DOI] [PubMed] [Google Scholar]
  15. Venetianer P., Leder P. Enzymatic synthesis of solid phase-bound DNA sequences corresponding to specific mammalian genes. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3892–3895. doi: 10.1073/pnas.71.10.3892. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yang R. C., Van de Voorde A., Fiers W. Specific cleavage and physical mapping of simian-virus-40 DNA by the restriction endonuclease of Arthrobacter luteus. Eur J Biochem. 1976 Jan 2;61(1):119–138. doi: 10.1111/j.1432-1033.1976.tb10003.x. [DOI] [PubMed] [Google Scholar]
  17. Zain B. S., Dhar R., Weissman S. M., Lebowitz P., Lewis A. M., Jr Preferred site for initiation of RNA transcription by Escherichia coli RNA polymerase within the simian virus 40 DNA segment of the nondefective adenovirus-simian virus 40 hybrid viruses Ad2 + ND 1 and Ad2 + ND 3 . J Virol. 1973 May;11(5):682–693. doi: 10.1128/jvi.11.5.682-693.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES