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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Stem Cells. 2015 Feb;33(2):601–614. doi: 10.1002/stem.1867

Figure 4. Labeled mTCs in alloreactive mice retain CFSE intensity in alloBMT mice receiving hMSCs.

Figure 4

(A) Volume rendering of CFSE fluorescence shows WP distribution of mTCs in syngeneic (“syn”), allogeneic (“allo”), and hMSC-infused allogeneic spleens (“allo + MSC”) at indicated time points post-transplant. (B) Probability density function (PDF) of CFSE fluorescence intensity per cell was used to estimate the fraction of bright mTCs over all detected mTCs. Green-shaded areas in histograms represent the percentages of mTC that retain high CFSE intensity (low mTC proliferation), while blue-shaded areas represent percentages of mTCs with low CFSE intensity (high mTC proliferation). Each representative histogram is from an individual mouse within the indicated experimental group (n=3 mice per experimental group). Data from one of two independent experiments is shown. (C) Quantitative analyses of spleen and white pulp volumes (in mm3) and the fraction of WP to total splenic volume (in %) are shown (n=3 mice per experimental group per time point; * p = 0.05, for indicated comparisons by Mann-Whitney test).