Table 1.
Primers | Primer sequence |
---|---|
For real-time PCR | |
QmiR-278-3p-F | 5′ACACTCCAGCTGGGTCGGTGGGACTTTCGT3′ |
QmiR-278-3p-R | 5′TGGTGTCGTGGAGTCG3′ |
QCYP6AG11-F | 5′CCCTACCTAGAGCAAGTTATCAA3′ |
QCYP6AG11-R | 5′ACGCCCTTTTCAATGTGGA3′ |
QACTIN-F | 5′AGCGTGAACTGACGGCTCTG3′ |
QACTIN-R | 5′ACTCGTCGTACTCCTGCTTGG3′ |
QU6-F | 5′GCTTCGGCTGGACATATACTAAAAT3′ |
QU6-R | 5′GAACGCTTCACGATTTTGCG3′ |
For vector constructs | |
293-CYP6AG11-F1 | 5′CGAGCTCGAGGCATCAGCGTGCTTACAA3′ |
293-CYP6AG11-R1 | 5′CCAAGCTTATTCAACCCCCGGTGGGT3′ |
293-CYP6AG11-R2 | 5′CCAAGCTTATTCAACCCCGGCTCGCT3′ |
C6/36-CYP6AG11-F | 5′GGACTAGTGAGATGGAAATGATTCTAACCATAACTCTCGTCG3′ |
C6/36-CYP6AG11-R | 5′CCCTCGAGCGTTTAGTTATCGCCTTATAGGTCAACC3′ |
F: Forward; R: Reverse