Figure 2. CD43 mediates cholesterol homeostasis.
(A) The bottom half of the aortas (n = 11 in each group) were subjected to lipid extraction by a modified Folch method with chloroform/methanol (2:1). Free (FC) and esterified cholesterol (CE) were separated by high-performance thin layer chromatography and quantified by scanning laser densitometry. FC and CE standards are shown on the left. (B) Cholesterol efflux to apoA-1 in primary control and CD43 deficient macrophages with and without AcLDL stimulation; (n=4 independent experiments). Data are expressed as mean ± SEM. **P<0.005