Table 1.
Survey of the 30 JCSG target proteins of Figure 3: GenBank accession codes, PDB accession codes, size, experimental conditions for APSY-NMR, extent of polypeptide backbone assignments
| GenBanka | PDBa | Size (aa) | Conc.b (mM) | Number of 2D APSY projections | NMR timec (h) | Percentage of residue assignment by UNIO-MATCH-2014d | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 5D HACACONH | 5D CBCACONH | 4D HACANH | C | P | E | U | |||||
| BC033015 | 2m51 | 62 | 1.2 | 26 | 26 | 31 | 13 | 79.1 | 17.7 | 0.0 | 3.2 |
| NP_346341.1 | 2m7o | 70 | 1.2 | 38 | 26 | 31 | 13 | 85.7 | 10.0 | 0.0 | 4.3 |
| BC008182 | 2lo1 | 71 | 1.5 | 26 | 32 | 27 | 13 | 85.9 | 12.7 | 0.0 | 1.4 |
| NM_00109834 | 2m34 | 71 | 0.8 | 42 | 42 | 31 | 16 | 78.9 | 15.5 | 5.6 | 5.6 |
| NP_254181.1 | 2mhg | 75 | 1.2 | 42 | 44 | 23 | 25 | 78.7 | 20.0 | 9.3 | 1.3 |
| NP_390345.1 | 2lyx | 87 | 1.2 | 32 | 26 | 25 | 23 | 77.0 | 16.1 | 8.0 | 6.9 |
| BC010264 | 2m7s | 90 | 1.2 | 20 | 20 | 23 | 17 | 74.4 | 18.9 | 4.4 | 6.7 |
| BC024153 | 2lxi | 91 | 1.2 | 32 | 28 | 31 | 27 | 54.9 | 38.5 | 7.7 | 6.6 |
| NM_002139 | 2mks | 92 | 1.2 | 38 | 41 | 29 | 38 | 87.0 | 10.9 | 0.0 | 2.1 |
| NM_184234 | 2mhn | 94 | 1.2 | 42 | 42 | 29 | 22 | 83.0 | 14.9 | 6.4 | 2.1 |
| BC008071 | 2m3d | 95 | 1.2 | 20 | 20 | 23 | 9 | 70.5 | 21.1 | 3.2 | 8.4 |
| NP_809759.1 | 2m4l | 99 | 1.2 | 20 | 20 | 23 | 9 | 71.7 | 21.2 | 4.0 | 7.1 |
| ZP_02034617.1 | 2lz0 | 100 | 1.0 | 30 | 30 | 27 | 13 | 84.0 | 13.0 | 1.0 | 3.0 |
| ZP_02042476.1 | 2mct | 102 | 1.2 | 40 | 38 | 29 | 46 | 92.1 | 5.9 | 1.0 | 2.0 |
| YP_002937094.1 | 2mca | 103 | 1.2 | 28 | 28 | 27 | 12 | 81.6 | 18.4 | 3.9 | 0.0 |
| BC043071 | 2m52 | 105 | 1.2 | 20 | 20 | 23 | 9 | 68.6 | 23.8 | 4.8 | 7.6 |
| YP_001298242.1 | 2mqc | 105 | 0.8 | 32 | 32 | 29 | 75 | 67.6 | 25.7 | 6.7 | 6.7 |
| YP_001714923.1 | 2mmb | 107 | 1.2 | 28 | 36 | 23 | 12 | 65.4 | 24.3 | 5.6 | 10.3 |
| ZP_02071672.1 | 2mhd | 110 | 1.2 | 32 | 30 | 27 | 12 | 73.6 | 16.4 | 2.7 | 10.0 |
| BC030493 | 2lq5 | 113 | 1.1 | 32 | 28 | 31 | 27 | 85.0 | 9.7 | 5.3 | 2.7 |
| ZP_02041089.1 | 2mc8 | 114 | 1.2 | 20 | 20 | 23 | 9 | 80.7 | 18.4 | 0.9 | 0.9 |
| YP_926445.1 | 2l6o | 114 | 1.2 | 42 | 42 | 27 | 15 | 84.2 | 8.8 | 7.0 | 0.9 |
| NP_809137.1 | 2mw1 | 118 | 1.2 | 38 | 38 | 29 | 28 | 79.7 | 16.9 | 3.4 | 5.1 |
| YP_001300941.1 | 2lrg | 126 | 1.2 | 42 | 38 | 23 | 55 | 92.1 | 7.1 | 0.8 | 0.8 |
| NP_390037.1 | 2lr4 | 128 | 1.2 | 32 | 32 | 27 | 37 | 89.1 | 9.4 | 1.6 | 1.6 |
| YP_001302112.1 | 2lge | 129 | 1.1 | 32 | 32 | 32 | 29 | 89.1 | 10.1 | 0.8 | 3.9 |
| NP_814968.1 | 2llg | 143 | 1.2 | 32 | 40 | 31 | 63 | 72.7 | 21.0 | 6.3 | 2.1 |
| ZP_02069618.1 | 2ml6 | 148 | 1.2 | 30 | 30 | 31 | 41 | 77.7 | 19.6 | 2.7 | 6.8 |
| YP_193882.1 | 2mwm | 151 | 1.2 | 28 | 28 | 23 | 66 | 80.8 | 9.9 | 9.3 | 1.3 |
| NP_372339.1 | 2mqb | 152 | 1.2 | 40 | 40 | 31 | 30 | 80.9 | 15.8 | 3.3 | 2.6 |
a
Proteins are identified by their GenBank accession codes and PDB accession codes.
b
Protein concentration of the NMR sample.
c
Total time used for acquiring the three APSY experiments (see text).
d
Percentage of residue assignment by UNIO-MATCH-2014. The letters C, P and E indicate the residues with complete, partial and erroneous assignments, respectively, and U represents the residues left unassigned (see also Fig. 3 and the text).