Figure 7. MB α’β’ GABA receptors promote nighttime sleep.

c305a-GAL4 was used to drive expression of Rdl-RNAi (A) or GABA_BR3-RNAi (B) in the α’β’ neurons. Top: shows total sleep in 30-min bins averaged across 3 days. Middle and bottom plots: show 3-day means of total sleep, mean sleep episode duration and number of sleep episodes quantified in 12-hr day/night bins. α’β’>Rdl-RNAi causes mild sleep loss and increases in nighttime sleep fragmentation, whereas α’β’>GABA_BR3-RNAi causes greater reductions in total sleep due to a decrease in the average sleep episode length. Grey shading indicates the dark period/night. All data are presented as mean ± SEM where * represents p < 0.05, **p < 0.001 and ***p < 0.0001 using the Mann-Whitney-Wilcoxon rank sum test. Statistics are described in the ‘Materials and methods’ section.

DOI: http://dx.doi.org/10.7554/eLife.03868.019

Figure 7—figure supplement 1. Sleep loss resulting from Rdl/GABA_BR3-RNAi is primarily due to MB α’β’ expression.

MB-GAL80 restricted c305a-GAL4 was used to drive Rdl-RNAi (A) or GABA_BR3-RNAi (B) in the c305a expression pattern outside of the MB. Top: shows total sleep in 30-min bins averaged across 3 days. Middle and bottom plots: show 3 day means of total sleep, mean sleep episode duration and number of sleep episodes quantified in 12-hr day/night bins. Non-MB c305a driven Rdl-RNAi results in a lesser degree of sleep loss and fragmentation and non-MB GABA_BR3-RNAi results in no changes in sleep or sleep structure, indicating these phenotypes are primarily due to MB α’β’ expression of c305a-GAL4. Grey shading indicates the dark period/night. All data are presented as mean ± SEM where * represents p < 0.05, **p < 0.001 and ***p < 0.0001 using the Mann-Whitney-Wilcoxon rank sum test. Statistics are described in the ‘Materials and methods’ section.

DOI: http://dx.doi.org/10.7554/eLife.03868.020