Table 2.

Kinetic parameters of rAaBGL1 for various natural substrates

Substrate	K m (mM)	k cat (s −1 )	k cat / K m (s −1 •mM −1 )
gentiobiose	0.52 ± 0.02	457 ± 4	873 ± 29
laminaribiose	0.41 ± 0.02	444 ± 17	1080 ± 20
laminaritriose	0.22 ± 0.01	337 ± 3	1550 ± 80
laminaritetraose	0.72 ± 0.03	304 ± 4	423 ± 11
laminaripentaose	1.13 ± 0.01	285 ± 5	251 ± 2
cellobiose	2.06 ± 0.07	354 ± 10	172 ± 3
cellotriose	0.45 ± 0.02	477 ± 3	1060 ± 50
cellotetraose	0.32 ± 0.01	433 ± 4	1340 ± 40
cellopentaose	0.41 ± 0.02	433 ± 9	1070 ± 30

Each value is the mean of triplicate experiments.