Figure 1.

Genome-wide transcript analysis of serotype emm59 group A Streptococcus (GAS) strains MGAS15252 (reference) and MGAS18055 (Mga H201R amino acid replacement). A: Growth curve of strains MGAS15252 and MGAS18055. Arrowheads indicate the 4 time points that were tested for gene transcript studies. B:mga expression is highest at late-logarithmic phase (OD₆₀₀ = 1.0). C: Principal component analysis is shown for genome-wide transcript profiles of strains grown to late-logarithmic phase. D: The expression of mga and seven Mga-regulated genes was significantly increased in strain MGAS18055 (H201R) compared to strain MGAS15252. Mean X-fold change in transcripts and genomic coordinates are shown for each gene. ^∗P < 0.05 compared to MGAS15252 (Baggerly's test after applying Bonferroni's correction for multiple comparisons).