Figure 3.

The Mga H201R amino acid replacement significantly increases strain virulence in a nonhuman primate model of joint infection. Cynomolgus macaques were inoculated in the right and left elbow with the isogenic wild-type and H201R strains, respectively, and observed for 7 days. A–D: Magnetic resonance imaging was used for noninvasive serial measurement of inflammation and edema. Representative T2 pseudocolored coronal maps collected on days 1 and 7 after inoculation are shown. E: The volume fraction of inflammation (VF) caused by each strain was calculated using thresholds 1 and 2, and disease was modeled using nonlinear least squares. F: Synovial fluid [colony-forming unit (CFU)/mL] was quantified. ^∗P < 0.05 compared to wild-type using repeated-measures analysis of variance; ^∗∗∗P < 0.001 versus wild-type using analysis of variance.