Table 1.

Probes and Primers Used for This Research

Primer	Sequence	Application
delMga A	5′-ATAGCGAGTGTTGCCATGTTAG-3′	Upstream fragment of mga for isogenic deletion
delMga B	5′-GTTATAGTTATTATAACATGTATTATGCATTAACTTCATGTCCTTATC-3′	Upstream fragment of mga for isogenic deletion
delMga C	5′-CTATTTAAATAACAGATTAAAAAAATTATAAACATCATCATAGGATTTCAGACGT-3′	Downstream fragment of mga for isogenic deletion
delMga D	5′-AATTTCCTCAGTCTTAGAGGCATCT-3′	Downstream fragment of mga for isogenic deletion
delMga E	5′-CATTTTCAAGAGCTAATGTTGGT-3′	Sequencing and PCR confirmation of deletion mga
delMga F	5′-TATACTTGTCGCTAGATTCTCT-3′	Sequencing and PCR confirmation of deletion mga
delMga G	5′-AGCTGCCTGCCTGTTGACCAATC-3′	Sequencing and PCR confirmation of deletion mga
delMga H	5′-ATCTAGCTTAGCTTGCAGATCAGTC-3′	Sequencing and PCR confirmation of deletion mga
Mga-spc forward	5′-GATAAGGACATGAAGTTAATGCATAATACATGTTATAATAACTATAAC-3′	Amplification of spec cassette
Mga-spc reverse	5′-ACGTCTGAAATCCTATGATGATGTTTATAATTTTTTTAATCTGTTATTTAAATAG-3′	Amplification of spec cassette
pDC-Mga top	5′-GGAAGATCTTAGAGTAATAGGTCAAATAATC-3′	Pmga-mga cloning into pDC
pDC-Mga bottom	5′-GGGAATCCATATGCTATGATGATGTTGCTTGC-3′	Pmga-mga cloning into pDC
pDC-Mga Top2	5′-ATGGTTCATACGGACTTG-3′	Sequencing of mga
pDC-Mga Top3	5′-TGCTATTAGTATCGTGACAAG-3′	Sequencing of mga
pDC-Mga Bottom2	5′-GATCAATCAGCTCACTT-3′	Sequencing of mga
H201R top	5′-GTAGATGTCAAAGTTCGTTTTACACTATTTCAG-3′	Site-directed mutagenesis to introduce H201R
H201R bottom	5′-CATCTACAGTTTCAAGCAAAATGTGATAAAGTC-3′	Site-directed mutagenesis to introduce H201R
mga qRT forward	5′-TCAATCAAGACCCGACATCA-3′	RT-qPCR analysis of mga
mga qRT reverse	5′-GGTCACGGCAACTTCGTATT-3′	RT-qPCR analysis of mga
mga Probe	5′-GCTCAATCTCAGCATCACCA-3′	RT-qPCR analysis of mga
tufA qRT forward	5′-CAACTCGTCACTATGCGCACAT-3′	RT-qPCR analysis of tufA
tufA qRT reverse	5′-GAGCGGCACCAGTGATCAT-3′	RT-qPCR analysis of tufA
tuf A probe	5′-CTCCAGGACACGCGGACTACGTTAAAAA-3′	RT-qPCR analysis of tufA

qPCR, real-time quantitative PCR.