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. 2015 Feb;185(2):485–495. doi: 10.1016/j.ajpath.2014.09.021

Figure 4.

Figure 4

Sp1 controls XT-1 promoter activity in nucleus pulposus (NP) cells. A and B: Effect of Sp1 inhibitors WP631 (A) and mithramycin A (Mith A) (B) on XT-1 promoter activity in rat NP cells. Both inhibitors significantly suppressed basal XT-1 promoter activity. C: Transfection of rat NP cells with increasing doses of DN-Sp1 suppressed XT-1 promoter. D and E: Overexpression of Sp1 (D) or Sp3 (E) did not influence the activity of XT-1 promoter in NP cells. F: Genomic chromatin immunoprecipitation analysis to detect Sp1 binding to XT-1 promoter in human NP cells. Pull down using Sp1 antibody showed significant enrichment of binding to Sp1 motifs at −227/−217 bp and −124/−114 bp over IgG alone. Negative primers (Neg) showed little or no amplification for experimental and IgG control samples. Values shown are means ± SEM of three independent experiments. ∗P < 0.05. DMSO, dimethyl sulfoxide.