Figure 1.

A Dual Effect of Chronic HCD Feeding on KRAS-Driven Lung Tumorigenesis

(A) Timetable of mouse treatments.

(B) Representative images of lung sections stained with hematoxylin and eosin (H&E) (inserts indicate area selected by arrows for higher magnification images; asterisk indicates the presence of nests of cells with large atypical nuclei).

(C) Histograms showing quantification of tumor burden, size, and number.

(D) Representative images of lung sections stained with anti-p-H3 (p-H3-positive cells in dark brown) and histograms indicating the percentage of p-H3-positive cells/tumor cells.

Data shown in (B)–(D) are from 29-week-old Kras^G12D mice treated as in (A) (females, n = 6–7/ group). Error bars represent SEM. Statistical analyses were done using one-way ANOVA (Tukey’s post test). ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. See also Figure S1.