Figure 2.
Unresolved ER Stress in Lung Tumors of HCD-Pre-Tumor-Onset KrasG12D Mice
(A) Gene set enrichment analysis (GSEA) plot showing chaperone enrichment score depicted by NES and nominal p value (the gene set used for GSEA is GO: 0006457).
(B) Xbp1s and Xbp1u mRNA levels.
(C) Immunoblot images and quantifications of cytosolic and nuclear XBP1 protein content (Lamin A and Tubulin were used as nuclear and cytosolic markers, respectively).
(D) mRNA levels of ER chaperones.
(E) Immunoblot images and quantification of ER chaperone protein content in total cell lysate.
(F) Immunoblot images and quantification of the phosphorylation status of eIF2α, IRE1α, and c-JUN.
(G) Chop mRNA levels.
All panels show data from microdissected lung tumors of 29-week-old KrasG12D males treated as described in Figure 1A (n = 5–6/group). Error bars represent SEM. Statistical analyses were done using two-tailed unpaired Student’s t test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. See also Figure S2.