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. 2014 May 19;29(6):1478–1491. doi: 10.1002/jbmr.2169

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© 2014 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals, Inc. on behalf of the American Society for Bone and Mineral Research

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Histological analysis of fracture calluses at days 14 and 21 days after fracture (safranin-O and fast green). (A) Representative micrographs from 14 and 21 days after fracture. (Top panel) Composite images of fracture calluses (×100 magnification). (B) Representative micrographs of trabecular and medullary tissues at 21 days after fracture (×200 magnification). (C) Graphic analysis showing the total callus area, total cartilage area, and total bone areas in µm² as calculated from the histomorphometric analysis for the control and B6.MRL-Fas^lpr/J strains at days 14 and 21 days after fracture. The mean value from 5 representative sections per callus (a total of 5 calluses were used per time point) are shown. The error bar is ± SD. Group differences are as denoted in Fig. 1. Statistical comparisons between strains times and interactions are as denoted by letters and are as presented in Figure legends 1 and 2. Significance was at p < 0.05.