Table 1.
E. coli strains and plasmids used in this study
| Strain | Characteristics | Reference |
|---|---|---|
| W3110 | Escherichia coli F−, λ−, INV(rrnD− rrnE)1 | ATCC 27325 |
| WPJ | W3110 transformed with plasmid pJLBaroG fbr tktA | This work |
| WPJRg | W3110 transformed with plasmid pJLBaroG fbr tktA and pTrcPALRg | This work |
| WPJAt | W3110 transformed with plasmid pJLBaroG fbr tktA and pTrcPALAt | This work |
| W(pheA−)Rg | W3110 (pheA::Km) transformed with plasmid pJLBaroG fbr tktA and pTrcPALRg | This work |
| W(pheA−)At | W3110 (pheA::Km) transformed with plasmid pJLBaroG fbr tktA and pTrcPALAt | This work |
| VH33 | Derivative of W3110 (ΔptsH, ptsI, crr::Km, ΔlacI, lacZ::loxP, PgalP::Ptrc) | [23] |
| VPJ | VH33 transformed with plasmid pJLBaroG fbr tktA | This work |
| VPJRg | VH33 transformed with plasmid pJLBaroG fbr tktA and pTrcPALRg | This work |
| VPJAt | VH33 transformed with plasmid pJLBaroG fbr tktA and pTrcPALAt | This work |
| Plasmids | ||
| pJLBaroG fbr tktA | aroG fbr under control of the lacUV5 promoter; and tktA under its native promoter, carries lacIq and tet genes | [26] |
| pTrc99A | Cloning vector, carries bla and lacIq genes and trc promoter | [27] |
| pTrcPALRg | PAL gene from R. glutinis gene cloned in pTrc99A | This work |
| pTrcPALAt | PAL gene from A. thaliana cloned in pTrc99A | This work |