(A) Foxp3 expression by CD4+ T cells from colonic lamina propria (LP) and spleens of WT and Niacr1−/− mice.
(B) Percent of Foxp3+ in CD4+ populations from colonic LP and spleens of WT and Niacr1−/− mice (n=3).
(C and E) IL-10 and IL-17 expression by (PMA+ionomycin)-stimulated colonic LP and splenic CD4+ T cells from WT and Niacr1−/− mice.
(D and F) Quantification of IL-10 and IL-17 producing CD4+ T cells shown in C and E respectively (n=3).
The numbers in A, C and E represent percentage of positive cells in indicated area. Error bars represent standard deviation (SD) of mean. *p<0.002. A representative of 3 independent experiments is shown.