Figure 3. TM domain separation is required for cell spreading.
(A) Stably transfected cells were seeded on immobilized Fbg (20μg/mL) with or without 1mM DTT for 30min at 37°C and visualized using differential interference contrast (DIC). Images are representatives from one of three independent assays. White bar: 10μm. (B) Average areas of adherent cells were quantified by pixel. The error bars represent S.D. from 100 randomly chosen cells.