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. 2015 Jan 24;10(1):e0116784. doi: 10.1371/journal.pone.0116784

Figure 5. Reduced levels of the miR-132/212 targets Ache, MeCp2 and Pnkd in the bladder following outlet obstruction.

Figure 5

Validated targets of miR-132/212 were examined at the protein level using western blotting at various times after surgical obstruction of the urethra (A). Summarized data (n = 6) for MeCP2, Ache and Pten is shown in panels B through D. Panel E shows double staining for MeCP2 (green) and DNA (blue) in control and obstructed (10 days) bladders. Panel F shows the percentage of MeCP2 positive nuclei in bladders from sham-operated and obstructed (10 days) rats (n = 6 and 7, respectively).