Table 3. Identification success for the best 100 bp and 50 bp mini-barcodes identified by sliding windows analysis in Spider.
Position* | Width, bp | Optimal threshold, % | Best Close Match | Success rate, % | |||
---|---|---|---|---|---|---|---|
ambiguous | correct | incorrect | no id | ||||
40 | 50 | 2.1 | 28 | 52 | 1 | 2 | 62.7 |
379 | 50 | 1.0 | 13 | 61 | 2 | 7 | 73.5 |
46 | 100 | 0.9 | 0 | 70 | 0 | 13 | 84.3 |
322 | 100 | 1.1 | 15 | 66 | 1 | 1 | 79.5 |
* position of the first nucleotide of the given window in the alignment.