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. 2015 Jan 24;10(1):e0117404. doi: 10.1371/journal.pone.0117404

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© 2015 Tinholt et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Syndecan-3 antigen levels (the 55 kDa monomeric form) in cell lysates from HCAECs Sum102 cells, and HCASMCs by Western blotting using anti-syndecan-3 and loading control (actin). One representative membrane of two is shown. Syndecan-3 protein band intensities (after normalization against loading control) relative to control cells are indicated for each cell type. Recombinant syndecan-3 (rSDC3) served as a positive control (~110 kDa). B) Cell surface associated syndecan-3 levels analyzed by flow cytometry in HCAEC cells. The histogram presents median fluorescence intensity obtained after syndecan-3 specific antibody labelling. Syndecan-3 knocked down cells (siSDC3); dark grey shaded, negative control siRNA; medium grey shaded and irrelevant control; light grey shaded. One representative experiment of two individual experiments (n = 4 biological parallels) is shown.