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. 2015 Jan 24;10(1):e0116852. doi: 10.1371/journal.pone.0116852

Figure 1. HDHB-depleted cells have impaired DNA repair and reduced sister chromatid exchange rate.

Figure 1

(A) Western blotting of HCT116 cells expressing short hairpin RNA (shRNA) against control (Ctl) or HDHB. (B) Left, example of micronuclei in cells exposed to 5 Gy ionizing radiation (IR). Right, percentage of cells with micronuclei. 300 cells from three independent experiments were counted. Error bars represent mean ± s.d. *P≤0.05, Student t-test. Arrows indicate micronuclei. (C) Colonies formed by surviving HCT116 cells treated with mitomycin C (MMC), or IR. Untreated cells were counted as 100% survival. The mean values ± s.d. from three independent experiments are plotted. (D) Sister chromatid exchange (SCE) of HCT116 cells expressing HDHB shRNA or control shRNA treated without mitomycin C (left) or with 100 ng/ml mitomycin C (right). Arrows indicate the exchange events. (E), (F) Analysis of SCE events in 100 cells expressing HDHB shRNA or control shRNA treated without mitomycin C (E) or with 100 ng/ml mitomycin C (F). The value at the top right of each panel is the mean ± s.d. SCE per cell from three independent experiments.