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. Author manuscript; available in PMC: 2016 Jan 30.
Published in final edited form as: Oncogene. 2014 Oct 20;34(31):4069–4077. doi: 10.1038/onc.2014.335

Figure 1. A screen for transcription factors regulating CBX7 expression identifies NR2E1/TLX.

Figure 1

(a) Scheme depicting the screen. A mouse CBX7 promoter luciferase reporter was co-transfected in HEK293T cells with a library of cDNAs of transcription factors to screen for cDNAs regulating CBX7 transcription. (b) Results of the screen. (c) Re-testing of candidates for CBX7 regulation identifies E2F family proteins and NR2E1 as activators of CBX7 transcription. (d) Cartoon showing the structure of NR2E1 wt and a DNA binding domain mutant (NR2E1 Δ40). DBD: DNA-binding domain; LBD: ligand binding domain. (e) NR2E1 induces CBX7 but not other CBX family genes. qRT-PCR for CBX family members in IMR90 cells infected with expression vectors for NR2E1 wt or NR2E1 Δ40 or with a control vector.