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. 2015 Jan 24;10(1):e0116641. doi: 10.1371/journal.pone.0116641

Figure 4. GAGs metabolism in normal and MPP+-stressed cells.

Figure 4

Analysis of enzymes involved in GAGs metabolism by Real Time PCR. Real time PCR of Hs2st, Hs3st1, Hs3st2, Hs3st3, Hs3st3A1, Hs3st3B1, Hs3st4, Hs3st5, Hs3st6, Hs6st1, Hs6st2vL, Hs6st2vS, Hs6st3, NDTS1, NDST2, NDST3, NDST4, Chst8 v1 and v2, Chst8 v3, Chst10, Chst11, Chst12, Chst14, epimerase and heparanase were performed with RNA extracts from MPP+-stressed (24 h) and normal cells. Values were normalized using geNorm software, an accurate normalisation of gene expression with multiple references genes [30]. The following reference genes glyceraldehyde 3-phosphate dehydrogenase (GAPDH), glutamine synthetase and alpha-tubulin were used. Each measure is the mean +/- S.E.M. from measurements performed on 3 independent cultures. * p<0.01 compared to control cells. Light grey bars: HS biosynthesis enzymes; dark grey bars: CS biosynthesis enzymes; white bar: HS degradation enzyme.