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. Author manuscript; available in PMC: 2015 Jan 25.
Published in final edited form as: Transplant Proc. 2013 Jun;45(5):2040–2044. doi: 10.1016/j.transproceed.2013.01.020

Fig. 1. Efficient knockdown of HPRT expression can be achieved by lentiviral gene transfer resulting in protection against 6TG.

Fig. 1

(A) Neuro-2a cells were transduced with lentiviral vectors expressing HPRT shRNA (RRL-HPRTshRNA) or no shRNA (control construct; RRL-ctr) at different MOI, and whole cell lysates were analyzed after transduction at the indicated time points by Western blot. Lane 1: mock, untransduced; Lane 2: ctr, MOI 10; Lane 3: HPRT shRNA, MOI 10; Lane 4: mock, untransduced; Lane 5: ctr, MOI 100; Lane 6: HPRT shRNA, MOI 100; Lane 7: HPRT shRNA, MOI 400. (B) At weekly intervals, Neuro-2a cells (untransduced (mock), or transduced with RRL-ctr or RRL-HPRTshRNA at MOI 100) were incubated with 0, 3 or 6 µM 6TG for 3 days before cell viability was measured by MTS assay. Cell viability values for cells treated with 3 µM 6TG (white bars) and 6 µM 6TG (black bars) were normalized to each respective set of untreated (0 µM 6TG) cells. (*: below limit of quantitation.)