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. Author manuscript; available in PMC: 2015 Jan 25.
Published in final edited form as: Nat Commun. 2012;3:1153. doi: 10.1038/ncomms2157

Figure 2. (A) Tyrosine sulfotransferase activity of the RaxST protein.

Figure 2

The reaction was initiated by adding the Y-peptide (DYQVSSPIYDINYYTSE) alone, RaxST protein alone, or both the Y-peptide and 0.5 µg of RaxST protein. (B) RaxST activity is specific to tyrosine peptides. The reaction was incubated with water, Y-peptide, A-peptide (DAQVSSPIADINAATSE), Dopamine, or Nitrophenol. (C) RaxST activity is specific to axY22. The reaction was initiated by adding axY22, axY22A (AENLSANFVEGDYVRTP), or water. NADPH production was measured at 340 nm using a spectrophotometer. Bars indicate the relative average value obtained for ΔA340/min ± standard deviation in three independent experiments. Different letters on the top of each bar indicate statistically significant differences between treatments. [(one way ANOVA analysis (Holm-Sidak test, P<0.05)].