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. Author manuscript; available in PMC: 2015 Jan 25.
Published in final edited form as: Nat Commun. 2012;3:1153. doi: 10.1038/ncomms2157

Figure 3. UV photodissociation mass spectrum of Tyr-sulfated peptide NLSYNFVE.

Figure 3

The Tyr-sulfated peptide was identified in the GluC digest of Ax21 after incubation with the sulfotransferase RaxST. The spectrum of the doubly deprotonated (2−) peptide was acquired on an Orbitrap Elite mass spectrometer equipped with an excimer laser (193 nm, 500 Hz, 5 ns pulse, 2 mJ per pulse, two pulses per spectrum). UVPD occurred in the higher-energy collisional dissociation (HCD) cell of the Orbitrap Elite mass spectrometer.