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SEM images of N. maculosa eggshell surface in top-view (a) and cross-section (b) following treatment with EDTA to remove the cuticle (see Material and methods for protocol). Images illustrate the rough pock-marked surface associated with reduced gloss. Arrow indicates the residual presence of the cuticle across a 1000 µm2 area of the eggshell illustrating how the cuticle fits on top of the rough pock-marked surface. Scale bars: (a) 10 µm; (b) 1 µm.
