Figure 3.

Immunohistochemical localization of ZBTB16 (A–D), SYCP3 (E–H), and GATA4 (I–L) in the seminiferous tubules at approximate stages of IV, VIII, and VII, respectively. (A, E, I) immunostaining with the antibodies indicated (red in A, E; green in I); (B, F, J) staining with DAPI (blue); and (C, G, K) merged pictures. (D, H, L) Staining with HE after fluorescent staining. (A–D) type A (A) spermatogonia were immunopositive, whereas intermediate (In) spermatogonia were immunonegative. (E–H) Preleptotene (Pl) spermatocytes and pachytene (P) spermatocytes were immunopositive. (I–L) Sertoli cells (Se) were immunopositive. Scale, 10 μm.