(A) Schematic of HCV genome and 10 proteins derived from the viral polyprotein.
(B) Flowchart of the strategy to generate the HCV-host protein-protein interactome.
(C) Anti-flag western blot (upper) and silver staining (lower) of 10 flag-tagged HCV proteins after transient transfection and affinity purification from Huh7 cells. Silver-stained bands corresponding to HCV bait proteins are indicated by arrows. See also Figure S1 and Tables S1, S2, S3.