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. Author manuscript; available in PMC: 2016 Jan 22.
Published in final edited form as: Mol Cell. 2015 Jan 22;57(2):329–340. doi: 10.1016/j.molcel.2014.12.028

Figure 1. Affinity-purification of HCV proteins and interactome strategy.

Figure 1

(A) Schematic of HCV genome and 10 proteins derived from the viral polyprotein.

(B) Flowchart of the strategy to generate the HCV-host protein-protein interactome.

(C) Anti-flag western blot (upper) and silver staining (lower) of 10 flag-tagged HCV proteins after transient transfection and affinity purification from Huh7 cells. Silver-stained bands corresponding to HCV bait proteins are indicated by arrows. See also Figure S1 and Tables S1, S2, S3.