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. Author manuscript; available in PMC: 2016 Jan 22.
Published in final edited form as: Mol Cell. 2015 Jan 22;57(2):329–340. doi: 10.1016/j.molcel.2014.12.028

Figure 2. A lentiviral shRNA screen identifies new HCV cofactors.

Figure 2

(A) Schematic of the HCV infection assay with an HCV-OFP (orange fluorescent protein mKO2) reporter virus.

(B) For each infection, we generated a standard curve of expected infection rates in scrambled shRNA-treated cells by plating the cells at indicated densities and obtaining the percentage of OFP-positive cells.

(C) Results of the HCV infection screen. Interacting host proteins that caused changes in infection levels are indicated on the y-axis. Relative infection levels (normalized to cell density) are indicated on the x-axis. The positive control, DGAT1, is shown in yellow, and the negative control, scrambled, in black. Results are shown as average (±SD) of a minimum of six experiments with two independent shRNAs (Experimental Procedures, S5). See also Figure S2 and Table S4.