(A) Localization of HCV-interacting host proteins as a pie chart. Each category contains the percentage of interactors of the total refined interactome that localize to a cell compartment. The heat map indicates the percentage of mitochondrial proteins within each individual bait protein's interactome.
(B) Differential centrifugation of HEK293T cells expressing GFP-flag, flag-core, or NS4B-flag to examine protein localization to mitochondria or cytosolic fractions. Control antibodies recognize calreticulin (endoplasmic reticulum), tubulin (cytosol) and COXIV (mitochondria).
(C) Immunoprecipitations with Huh7 cell lysates expressing GFP-flag or NS4B-flag were performed with α-flag affinity resin and blotted with the indicated antibodies to demonstrate that NS4B specifically interacts with endogenous CYB5B, a mitochondrial outer membrane protein. See also Figure S3.