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. Author manuscript; available in PMC: 2016 Jan 22.
Published in final edited form as: Mol Cell. 2015 Jan 22;57(2):329–340. doi: 10.1016/j.molcel.2014.12.028

Figure 4. Cellular localization of HCV-interacting host proteins.

Figure 4

(A) Localization of HCV-interacting host proteins as a pie chart. Each category contains the percentage of interactors of the total refined interactome that localize to a cell compartment. The heat map indicates the percentage of mitochondrial proteins within each individual bait protein's interactome.

(B) Differential centrifugation of HEK293T cells expressing GFP-flag, flag-core, or NS4B-flag to examine protein localization to mitochondria or cytosolic fractions. Control antibodies recognize calreticulin (endoplasmic reticulum), tubulin (cytosol) and COXIV (mitochondria).

(C) Immunoprecipitations with Huh7 cell lysates expressing GFP-flag or NS4B-flag were performed with α-flag affinity resin and blotted with the indicated antibodies to demonstrate that NS4B specifically interacts with endogenous CYB5B, a mitochondrial outer membrane protein. See also Figure S3.