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. 2005 May 7;11(17):2597–2602. doi: 10.3748/wjg.v11.i17.2597

Figure 5.

Figure 5

SDS-PAGE analysis of eluted fractions from the DEAE column by different elution buffers. After fermentation, the supernatant of SMD1168/pPIC9-IFNα2b-(G4S)2-Tα1 was collected and loaded on the DEAE column. The fractions eluted sequentially by elution buffer A and B were analyzed by SDS-PAGE. Lane1-8: the fractions subsequently eluted by elution buffer A (0.05 mol/L Tris-HCl, 0.10 mol/L NaCl, pH 8.0); lane 9-14: the fractions subsequently eluted by elution buffer B (0.05 mol/L Tris-HCl, 0.10 mol/L NaCl, pH 8.0).